5M甜菜堿溶液-PCR級(jí)
5MBetaine Solution-PCR級(jí)
● 產(chǎn)品貨號(hào)及規(guī)格：
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貨號(hào)
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名稱(chēng)
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規(guī)格
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貯存
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RTE3103-02
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5M 甜菜堿溶液
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5×1 ml
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-20℃
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● 產(chǎn)品簡(jiǎn)介：
Betaine, DMSO and formamide helps in reducing the secondary
structure of GC rich templates and assists amplification of these templates.
Betaine is used at3.5Mto0.1M, DMSO should be used between 2-8%,
however 10% DMSO can reduce Taq polymerase activity by up to 50%. Formamide is
generally used at 1-5%. It has been our experience that the use of DMSO in
combination with betaine is superior to using formamide.
● 貯存：-20℃貯存。有效期一年。
● 使用說(shuō)明：
1．-20℃保存的甜菜堿溶液常溫融化，混勻。
2. 根據(jù)根據(jù)PCR反應(yīng)體系，加入適量體積的5M甜菜堿溶液。
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20 μl 體系
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反應(yīng)體系終濃度
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10×PCR Buffer
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2 μl
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1×
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5M Betaine*
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4 μl
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1 M
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模板
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0.05-0.5μg
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as you wish
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上游引物 10μM
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0.5 μl
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0.2 μM
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下游引物 10μM
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0.5 μl
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0.2 μM
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dNTP10mM
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0.5 μl
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200 μM
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Taq
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0.5-1 μl
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2.5-5 U
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滅菌水
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up to 20 μl
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注*：不同模板，不同片段的擴(kuò)增可能需要不同濃度的甜菜堿，可以適當(dāng)調(diào)節(jié)加入體積，找到適合自己反應(yīng)的甜菜堿濃度。
● References：
1 Rees, William A., et al., Betaine can eliminate the
base pair composition dependence of DNA melting. Biochemistry, 32, 137-144 (1993)